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. 2020 Dec 4;11:6221. doi: 10.1038/s41467-020-20099-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Cross-section of 4-day-old cotyledon epidermal cell expressing mNeonGreen-mPTS^PEX26 (green) and mRuby3-PTS1 (magenta) shows peroxisomal ILVs enclosing (yellow arrowhead) or lacking (blue arrowheads) PTS1 reporter. b, c Cross-sections of 4-day-old cotyledon epidermal cells expressing mRuby3-PTS1 (magenta), mTagBFP2-SCO3 (blue), and mNeonGreen-mPTS^PEX22 (green) (b) or mPTS^PEX26-mNeonGreen (green) (c) show SCO3 accumulation (arrowheads) in a subset of ILVs. d, e Cross-sections of 4-day-old cotyledon epidermal cells expressing mRuby3-PTS1 (magenta), mTagBFP2-UP9 (blue), and mNeonGreen-mPTS^PEX22 (green) (d) or mPTS^PEX26-mNeonGreen (green) (e) show UP9 accumulation (arrowheads) in a subset of ILVs. f Cross-section of 3-day-old cotyledon epidermal cells expressing mNeonGreen-mPTS^PEX26 and mRuby3-PTS1 (magenta) shows ILVs with (green) and without (arrowheads) mNeonGreen-mPTS^PEX26 membrane fluorescence.