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. 2020 Dec 4;11:6224. doi: 10.1038/s41467-020-20010-9

Fig. 6. Regulators of morphogenesis influence chlamydosporulation through Rme1.

Fig. 6

a C. albicans hog1ΔΔ and efg1ΔΔ mutant strains expressing PTDH3-RME1 and PTET-RME1 respectively were grown during 3 days under chlamydospore-inducing conditions on PCB plates, b the same cells recovered from cellophane film on PCB plates expressing either the CSP1-GFP (left) or PPGA55-GFP (right) fusions. The cultures were inspected by phase contrast and fluorescence microscopy. Doxycycline was added when needed to a final concentration of 40 μg/mL. Scale bar = 10 μm. c C. albicans sfl1ΔΔ and ndt80ΔΔ mutant strains in both SC5314 (CEC5288 and CEC5290, respectively, top panels) or CEC2018 (CEC5284 and CEC5286, respectively, bottom panels) backgrounds were grown overnight in liquid chlamydospore-inducing conditions. Scale bar = 10 μm. d Strains constitutively overexpressing either SFL1 (middle panels) or NDT80 (right panels) in the WT backgrounds of SC5314 (CEC5278 and CEC5280, respectively, top) or CEC2018 (CEC5274 and CEC5276, respectively, bottom) cultured overnight in liquid chlamydospore-inducing conditions; SC5314 and CEC2018 transformed with the empty overexpression plasmid (CEC5393 and CEC5292, respectively) were used as controls (left panels). Strains were examined by light microscopy for their ability to form chlamydospores (white arrowheads in (c) and (d)). Scale bar = 10 μm.