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. 2020 Dec 4;10:21226. doi: 10.1038/s41598-020-78210-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Virus detection in oropharyngeal swabs and organ samples in chickens and turkeys. Detection of viral RNA in inoculated chickens in oropharyngeal swabs (A) or organs (nasal cavity) (B) at 4 dpi was determined by RT-qPCR targeting the M gene and are expressed as equivalent Log₁₀ PFU/ml. Virus excretion in oropharyngeal swabs (C,D) as well as in brain (E) and nasal cavity (F) at 4 dpi was determined by RT-qPCR targeting the M gene and are expressed as equivalent Log₁₀ PFU/ml. Asterisk indicates significant difference compared to K319.