Table 2.
Clinical examination of turkeys after oculonasal inoculation.
| PI | Inoculated Turkeys | Contact Turkeys | |||||
|---|---|---|---|---|---|---|---|
| Morbidity | Shedding | Seroconversion | Morbidity | Shedding | Seroconversion | ||
| K319 | 0.4 | 9/10* | 9/10 | 7/7 | 3/5 | 5/5 | 5/5 |
| K319G | 0.1 | 1/10 | 10/10 | 7/7 | 1/5 | 5/5 | 5/5 |
| K319A | 0.1 | 2/10 | 8/10 | 7/7 | 1/5 | 5/5 | 5/5 |
| K319N | 0.6 | 10/10 | 10/10 | 7/7 | 2/5 | 5/5 | 5/5 |
| K319S | 0.1 | 3/10 | 9/10 | 7/7 | 1/5 | 4/5 | 4/5 |
| K319D | 0.4 | 9/10 | 10/10 | 7/7 | 4/5 | 3/5 | 5/5 |
* Number of positive birds/total examined.
Turkeys were challenged with 105.7 pfu/bird and 1 dpi 5 birds were added to assess transmission. At 4 dpi, 3 directly-inoculated turkeys were euthanized to assess virus distribution and lesions in different organs. Seroconversion (number of positive birds / total examined) was tested at 10 dpi using ELISA. Clinical scoring was conducted as recommended by the OIE on a scale 0 to 3: 0 = apparently healthy, 1 = birds showed 1 clinical sign (ruffled feather, respiratory disorders, diarrhea), 2 = birds showed more than 1 clinical signs and 3 = dead birds. The pathogenicity index (PI) is the mean of all clinical scores for all inoculated birds in 10 day-observation period. Clinical examination was done blindly by two veterinarians. Shedding was determined by RT-qPCR.