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. 2020 Dec 4;10:21280. doi: 10.1038/s41598-020-77978-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Enhanced ERK signaling in 3T3/L1 cells by BR103354. (a) Fully differentiated 3T3/L1 adipocytes were incubated with full-length hFGF21 at 500 ng/mL. FGF21 was pre-incubated with PBS or recombinant FAP at 100 ng/mL (substrate: enzyme ratio = 5:1) for 2 h, with or without 5 µM of BR103354. Phosphorylated ERK (p-ERK), total ERK (ERK), Glut1, adiponectin and β-actin were measured by immunoblots. (b) Quantification of p-ERK, Glut1 and adiponectin was obtained with densitometric analysis, and normalized with total ERK or β-actin, respectively. All data are the means ± S.D. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. no treatment (control) group.