Figure 4. SLC25A51 is sufficient for transport of NAD⁺ into yeast mitochondria lacking their endogenous transporters, NDT1 and NDT2.

³H-NAD⁺ uptake measured in isolated mitochondria from wildtype (n=4 independent experiments), NDT1 and NDT2 double knockout (DKO) (n=4 independent experiments), and a, DKO + overexpression of SLC25A51 (n=4 independent experiments) or b, DKO + overexpression of SLC25A52 (n=3 independent experiments) yeast; grey line indicates mean basal radioactivity in DKO samples. P values were determined by a two-way ANOVA with multiple comparisons analysis using Dunnett’s method. c, Initial rates of NAD⁺ uptake into isolated yeast mitochondria (n=4 independent experiments for WT, DKO, and DKO + SLC25A51; n=3 for DKO + SLC25A52). d, NAD⁺ content measured from isolated yeast mitochondria (n=3 biological independent replicates). P values were determined by one-way ANOVA with multiple comparisons analysis using Tukey’s method. Data represented as mean ± SEM. **P<0.01, and ***P<0.001 vs DKO, ^##P<0.01 and ^###P<0.001 vs wildtype (exact P values are provided in the source data).