Extended Data Table 1. Essential mitochondrial solute carrier family 25 genes determined by genome-wide CRISPR/Cas9 screens examining cellular viability.
Table includes essential gene name, number of cell lines in which gene is essential, number of cell lines tested, corresponding citation of study utilizing genome-wide CRISPR/Cas9 screens to determine essential genes, annotation (Uniprot), and tissue expression profile (Human Protein Atlas 20). #SLC25A51 was not tested in Hart et al. Cell, 2015. Refs. 6, 7, 22, 23.
| Essential SLC25A Genes | Cell lines where gene is essential/ Cell lines tested | Citation | Annotation | Distribution of Expression in Tissues |
|---|---|---|---|---|
| SLC25A1 | (2/4) | Wang et al. Science, 2015 | Mitochondrial Tricarboxylate Transport Protein | All tissues |
| (1/1) | Bertomeu et al. MCB, 2017 | |||
| SLC25A3 | (1/4) | Wang et al. Science, 2015 | Mitochondrial Phosphate Carrier Protein | All tissues; Enriched in Heart and Skeletal Muscle |
| (1/1) | Bertomeu et al. MCB, 2017 | |||
| (2/2) | Blomen et al. Science, 2015 | |||
| (2/5) | Hart et al. Cell, 2015 | |||
| SLC25A5 | (1/2) | Blomen et al. Science, 2015 | ADP/ATP translocase 2 | All tissues |
| SLC25A10 | (5/5) | Hart et al. Cell, 2015 | Mitochondrial Dicarboxylate Carrier | All tissues; Enriched in liver |
| SLC25A14 | (1/4) | Wang et al. Science, 2015 | Mitochondrial Uncoupling Protein 5 | All tissues |
| SLC25A19 | (1/1) | Bertomeu et al. MCB, 2017 | Mitochondrial Thiamine Pyrophosphate Carrier | All tissues; Enriched in testis |
| (2/2) | Blomen et al. Science, 2015 | |||
| (2/5) | Hart et al. Cell, 2015 | |||
| SLC25A22 | (2/5) | Hart et al. Cell, 2015 | Mitochondrial Glutamate Carrier 1 | All tissues; Enriched in brain and pancreas |
| SLC25A25 | (1/5) | Hart et al. Cell, 2015 | Calcium-binding Mitochondrial Carrier Protein SCaMC-2 | All tissues |
| SLC25A26 | (1/4) | Wang et al. Science, 2015 | Mitochondrial S-Adenosylmethionine Carrier Protein | All tissues |
| (1/1) | Bertomeu et al. MCB, 2017 | |||
| (2/2) | Blomen et al. Science, 2015 | |||
| (3/5) | Hart et al. Cell, 2015 | |||
| SLC25A28 | (4/5) | Hart et al. Cell, 2015 | Mitoferrin-2 | All tissues |
| SLC25A29 | (1/5) | Hart et al. Cell, 2015 | Mitochondrial Basic Amino Acids Transporter | All tissues; Enhanced in thyroid gland |
| SLC25A31 | (1/5) | Hart et al. Cell, 2015 | ADP/ATP translocase 4 | Enriched in testis |
| SLC25A32 | (1/4) | Wang et al. Science, 2015 | Mitochondrial Folate Carrier | All tissues |
| SLC25A39 | (1/5) | Hart et al. Cell, 2015 | Unannotated; Heme biosynthetic process and transmembrane transport | All tissues |
| SLC25A41 | (1/5) | Hart et al. Cell, 2015 | Unannotated; ATP transmembrane transporter activity | Detected in some; Enriched in brain and testis |
| SLC25A42 | (2/5) | Hart et al. Cell, 2015 | Mitochondrial Coenzyme A Transporter | All tissues; Enriched in liver |
| SLC25A43 | (1/4) | Wang et al. Science 2015 | Unannotated; ATP transmembrane transporter activity | All tissues |
| SLC25A45 | (1/4) | Wang et al. Science, 2015 | Unannotated; Acyl carnitine transmembrane and ATP transmembrane transporter activity | All tissues; Enriched in pancreas |
| (3/5) | Hart et al. Cell, 2015 | |||
| SLC25A51* | (3/4) | Wang et al. Science, 2015 | Unannotated; No prediction | All tissues |
| (1/1) | Bertomeu et al. MCB, 2017 | |||