Skip to main content
. Author manuscript; available in PMC: 2021 Mar 14.
Published in final edited form as: Nat Struct Mol Biol. 2020 Sep 14;27(12):1105–1114. doi: 10.1038/s41594-020-0506-5

Extended Data Fig. 1. Analysis of DNA sizes in control circular DNA, control linear DNA and 3C library DNA before and after exonuclease digestion.

Extended Data Fig. 1

Agarose gel analysis (0.8% agarose) of DNA size of control circular plasmid DNA, control linear plasmid DNA and of 3C library DNA before and after exonuclease digestion. 3C library and control plasmid DNA were digested with Exonuclease V and T5 exonuclease to determine whether 3C ligation products are linear or circular. In the presence of either exonuclease dsDNA will be digested, and circular DNA will remain present. As control both exonucleases are shown not to digest circular plasmid (pCMV6, 4.6kb) (lane 1-3), whereas linearized plasmid was digested (lane 4-6). Different concentration of 3C library and ligase did not result in detection of circular ligation products as no DNA was detected after exonuclease treatment (lanes 7-18). Markers: 1 kb ladder (New England Biolabs).