KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal antibody anti-Ki-67 | Dako | Cat# M7240, RRID:AB_2142367 |
| Mouse monoclonal antibody anti-beta-Actin | Sigma-Aldrich | Cat# A1978, RRID:AB_476692 |
| Rabbit monoclonal antibody anti-NRF2 | Cell Signaling Technology | Cat# 12721, RRID: AB_2715528 |
| Rabbit monoclonal antibody anti-GPX4 | Abcam | Cat# ab125066, RRID: AB_10973901 |
| Rabbit monoclonal antibody anti-TSC1 | Cell Signaling Technology | Cat# 6935, RRID: AB_10860420 |
| Mouse monoclonal antibody anti-ACSL4 | Santa Cruz Biotechnology | Cat# sc-271800, RRID: AB_10715092 |
| Rabbit monoclonal antibody anti-TRXR1 | Cell Signaling Technology | Cat# 15140, RRID: AB_2798725 |
| Rabbit monoclonal antibody anti-S6 Ribosomal Protein (RPS6) | Cell Signaling Technology | Cat# 2217, RRID: AB_331355 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAPI | Sigma-Aldrich | Cat# D9542, CAS: 28718-90-3 |
| Doxycycline hydrochloride | Sigma-Aldrich | Cat# D3447, CAS: 10592-13-9 |
| ML210 | Sigma-Aldrich | Cat# SML0521, CAS: 1360705-96-9 |
| RSL3 | Sigma-Aldrich | Cat# SML2234, CAS: 1219810-16-8 |
| Torin1 | Tocris | Cat# 4247 CAS: 1222998-36-8 |
| Ferrostatin-1 | Sigma-Aldrich | Cat# SML0583, CAS: 347174-05-4 |
| BODIPY™ 581/591 C11 | Invitrogen | Cat# D3861 |
| Roche cOmplete, EDTA-free | Sigma-Aldrich | Cat#11873580001 |
| Pierce Trypsin Protease, MS Grade | Thermo Fisher | Cat# 90058 |
| Lys-C, Mass Spectrometry Grade | Wako Chemicals | Barcode#4987481427648 |
| TMT10plex Isobaric Label Reagent Set plus TMT11-131C Label Reagent | Thermo Fisher | Cat# A34808 |
| Critical Commercial Assays | ||
| Q5® Site-Directed Mutagenesis Kit | NEB | Cat# E0554S |
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent | Cat# 200522 |
| ROS-Glo™ H2O2 Assay | Promega | Cat# G8821 |
| OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit | Cell Biolabs | Cat# STA-344 |
| GSH/GSSG-Glo™ Assay | Promega | Cat# V6611 |
| Iron Assay Kit | Abcam | Cat# ab83366 |
| Deposited Data | ||
| RNAseqV2, mutation and copy number | The Cancer Genome Atlas | https://www.cancer.gov/tcga |
| Functional genomic screening data | Project Achilles | https://depmap.org/portal/achilles/ |
| Small-molecule sensitivity data | Genomics of Drug Sensitivity in Cancer | https://www.cancerrxgene.org/ |
| Small-molecule sensitivity data | The Cancer Therapeutics Response Portal | https://portals.broadinstitute.org/ctrp/ |
| Mass spectrometry proteomics data for A549 and H1437 cells with or without NRF2 knockdown | This paper | ProteomeXchange Consortium (https://www.proteomexchange.org), Identifier: PXD018634 |
| Experimental Models: Cell Lines | ||
| H522 | ATCC | Cat# CRL-5810, RRID: CVCL_1567 |
| H1975 | ATCC | Cat# CRL-5908, RRID: CVCL_1511 |
| H226 | ATCC | Cat# CRL-5826, RRID: CVCL_1544 |
| SW 900 | ATCC | Cat# HTB-59, RRID: CVCL_1731 |
| H1568 | ATCC | Cat# CRL-5876, RRID: CVCL_1476 |
| H1838 | ATCC | Cat# CRL-5899, RRID: CVCL_1499 |
| H23 | ATCC | Cat# CRL-5800, RRID: CVCL_1547 |
| H358 | ATCC | Cat# CRL-5807 RRID: CVCL_1559 |
| H596 | ATCC | Cat# HTB-178 RRID: CVCL_1571 |
| H520 | ATCC | Cat# HTB-182 RRID: CVCL_1566 |
| SK-MES-1 | ATCC | Cat# HTB-58 RRID: CVCL_0630 |
| H1792 | ATCC | Cat# CRL-5895, RRID: CVCL_1495 |
| H2228 | ATCC | Cat# CRL-5935, RRID: CVCL_1543 |
| A549 | ATCC | Cat# CCL-185, RRID: CVCL_0023 |
| H1437 | ATCC | Cat# CRL-5872, RRID: CVCL_1472 |
| H460 | ATCC | Cat# HTB-177, RRID: CVCL_0459 |
| H1299 | ATCC | Cat# CRL-5803, RRID: CVCL_0060 |
| H2030 | ATCC | Cat# CRL-5914, RRID: CVCL_1517 |
| H2122 | ATCC | Cat# CRL-5985, RRID: CVCL_1531 |
| H1944 | ATCC | Cat# CRL-5907, RRID: CVCL_1508 |
| Oligonucleotides | ||
| Primers for the construction of NFE2L2 mutants: listed in Table S7 | This paper | N/A |
| Primers for the construction of LentiCRISPR plasmids: listed in Table S7 | This paper | N/A |
| Primers for quantitative PCR: listed in Table S7 | This paper | N/A |
| Primers for amplification of sgRNA: listed in Table S7 | This paper | N/A |
| Recombinant DNA | ||
| TRIPZ-inducible lentiviral shNRF2-#1 | Dharmacon | V3THS_306092 |
| TRIPZ-inducible lentiviral shNRF2-#2 | Dharmacon | V3THS_306096 |
| Inducible lentiviral non-silencing shRNA | Dharmacon | RHS4743 |
| TRC lentiviral shNRF2-#1* | Dharmacon | TRCN0000007555 |
| TRC lentiviral shNRF2-#2* | Dharmacon | TRCN0000007558 |
| shRNA against GFP | Addgene (Sancak et al., 2008) | Plasmid #30323 |
| NFE2L2-pLX304 | Dharmacon | OHS6085-213573755 |
| NFE2L2-pLX304 mutants | This paper | N/A |
| LentiCRISPR plasmids | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7.0 | GraphPad Prism Software | N/A |
| RStudio | RStudio | N/A |
| Database searching and reporter ion quantitation in-house software | Huttlin et al., 2010 | NA |
| Sequest algorithm | Eng et al., 1994 | NA |
| R | https://cran.r-project.org/ | Version 3.5.1 |
| MAGeCK-VISPR | Li et al., 2015 | Version 0.5.6 |
| Other | ||
| Waters 200mg Sep-Pak | Waters | Prod#WAT054945 |
| Orbitrap Fusion | Thermo Fisher | IQLAAEGAAPFADBMBCX |