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. Author manuscript; available in PMC: 2021 Apr 9.
Published in final edited form as: Oncogene. 2020 Oct 9;39(49):7209–7223. doi: 10.1038/s41388-020-01493-8

Fig. 8. Upregulation of miR-375 and miR-301a drive neuroendocrine differentiation states in prostate cancer.

Fig. 8.

A. Real time PCR analyses of miR-375 (left panel) and miR-301a (right panel) in CRPC-NE (LuCaP 49, 145.1 and 145.2) vs CRPC-Adeno PDX models (LuCaP 70, 78, 81 and 92). RNU48 was used an endogenous control.

B. Real time PCR analyses of miR-375 (left panel) and miR-301a (right panel) in BPH1 and PCa cell lines (PC3, LNCaP, LNCaP-AR, LNCaP-AR-Enzalutamide resistant, NCI-H660). RNU48 was used as an endogenous control.

C. LNCaP and C42B cells were transinetly transfected with control/miR-375/miR-301a mimics for 72 hours followed by functional assays. Real time PCR analyses of miR-375 and miR-301a expression in control/miR-375 transfected or control/miR-301a transfected LNCaP and C42B cells. RNU48 was used an endogenous control.

D. In vitro migration (left panels) and invasion assays (right panels) in control/miR-375 (upper panels) and control/miR-301a-transfected (lower panels) LNCaP and C42B cells.

E. Left panel: Real time PCR analyses of ENO2 and SYP expression in miR-CON/miR-375 transfected LNCaP and C42B cells. GAPDH was used as an endogenous control. Right panel: Western blot analyses of CHGA and ENO2 in miR-CON/miR-375 transfected LNCaP and C42B cells. GAPDH was used as a loading control.

F. Western blot analyses of Akt and Src in miR-CON/miR-375 transfected LNCaP and C42B cells. GAPDH was used as a loading control.

G. Real time PCR analyses of ENO2 and AR expression in miR-CON/miR-301a transfected LNCaP and C42B cells. GAPDH was used as an endogenous control.

H. Western blot analyses of CHGA, ENO2 and AR in miR-CON/miR-301a transfected LNCaP and C42B cells. GAPDH was used as a loading control.