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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Cancer Res. 2020 Oct 22;80(23):5408–5423. doi: 10.1158/0008-5472.CAN-20-0831

Figure 1: Metabolic autofluorescence imaging of macrophages in the Stacks 3D microscale co-culture system.

Figure 1:

Illustration of experimental workflow showing (A) design of the Stacks 3D co-culture of macrophages/monocytes and tumor cells from primary patient samples, along with cell lines of mouse and human origin, (B) validation of metabolic autofluorescence imaging in 2D culture with standard stimulations and in Stacks 3D microscale system with macrophages alone, and (C) metabolic autofluorescence imaging of 3D migration and single-cell metabolism for macrophages in the Stacks 3D microscale co-culture system.