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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Nature. 2020 Oct 14;588(7836):112–117. doi: 10.1038/s41586-020-2821-8

Extended Data Figure 6. ulti-color in situ hybridization for anatomical validation of transcriptomic cell-types.

Extended Data Figure 6.

M a, Quantification of SFO Htr7- and Rxfp1-positive cells and their overlap in the SFO (n=12 slices from 4 animals). Scale bar 20 μm. Nuclei are visualized by DAPI staining (white). b, Quantification of Bmp3- and Rxfp1-positive cells and their overlap in the OVLT (n=15 slices from 8 animals). Scale bar 20 μm. c, Rxfp1-and Pdyn-positive cells co-express c-Fos under water deprived conditions. Representative images from 8, 4, 8 and 4 sections from 2 independent experiments for SFO Rxfp1/c-Fos, OVLT Rxfp1/c-Fos, SFO Pdyn/c-Fos and OVLT Pdyn/c-Fos stains respectively. Scale bar 10 μm. d, Cell types labeled by Rxfp3 (SFO) and Cpne4 (OVLT) express c-Fos under osmotic thirst conditions (left). Cell types labeled by Htr7 (SFO) and Bmp3 (OVLT) express c-Fos under hypovolemic thirst (right). Representative images from 2, 2, 3 and 2 sections from 2 independent experiments for SFO Rxfp3/c-Fos, OVLT Cpne4/c-Fos, SFO Htr7/c-Fos and OVLT Bmp3/c-Fos stains respectively. Scale bar 10 μm.