Extended Data Fig. 8 |.

a, The fluorescence signal generated from unregulated ROSALIND reactions are stable over weeks. The increase in fluorescence from Day 1 is likely due to a concentration increase caused by evaporation when the plate was taken out of the incubator after the first measurements. b, The shelf-stability of freeze-dried ROSALIND reactions (unregulated, TetR-regulated, and aTc-induced) decay over the course of a month without proper packaging. c, Packaging of freeze-dried ROSALIND: 1) reactions are lyophilized overnight, 2) the overnight lyophilized reactions are purged with inert gas such as argon, and 3) the reactions are placed into a light-protective bag with a desiccant and immediately impulse heat sealed (Supplementary Video 2). d, When this packaging method is implemented, lyophilized reactions are functional out to 2.5 months. Though we observed signal decay, the signal from rehydrated reactions after 2.5 months is clearly visible. Images are shown for one replicate with other replicate images included in Supplementary Data File 1. Unregulated reactions were lyophilized with 25 nM of the 3WJdB template, and TetR-regulated reactions with additional 1.25 µM TetR dimer along with the components of IVT specified in the In vitro transcription reactions method section. Unregulated and TetR-regulated reactions were then rehydrated with laboratory-grade water, and aTc-induced reactions were rehydrated with 10 µM of aTc. All data shown for n=3 independent biological replicates as points with raw fluorescence values standardized to MEF (µM FITC), and center values representing averages of the replicates. Error bars indicate the average value of 3 independent biological replicates ± standard deviation. The original, uncropped images shown in c and d can be found in Supplementary Data File 1.