Fig. 3 |. ROSALIND is modular and can be used to sense complex molecules, small molecules and metal ions.

TetR can be used to sense a, tetracycline, b, doxycycline and other tetracycline family antibiotics. c, Oxytetracycline sensing with OtrR. d, Chlortetracycline sensing with CtcS. MphR senses the macrolides e, erythromycin, f, azithromycin, g, clarithromycin and h, roxithromycin. i, 3-hydroxy benzoic acid sensing with MobR. j, Benzalkonium chloride sensing with QacR. k, Naringenin sensing with TtgR. l, Uric acid sensing with HucR. m, Zn²⁺ sensing from ZnSO₄ with SmtB. n, Cu²⁺/Cu⁺ sensing from CuSO₄ with CsoR from Bacillus subtilis. o, Pb²⁺ sensing from PbCl₂ and p, Cd²⁺ sensing from CdCl₂ with CadC. Each reaction contains the indicated amount of ligand (+) dissolved in lab-grade water, ethanol, Tris-base buffer, dimethyl sulfoxide, or diluted sodium hydroxide, or a lab-grade water control (−) (Supplementary Table 2). All data shown for n=3 independent biological replicates as lines with raw fluorescence values standardized to MEF (µM FITC). Shading indicates the average value of 3 independent biological replicates ± standard deviation. DNA template and aTF concentrations used in each reaction can be found in Supplementary Table 2.