Extended Data Fig. 1 |.

Homemade in vitro transcription reactions were compared to a commercially available high yield transcription kit (NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit). 25 nM DNA encoding T7-3WJdB-T was added to each reaction in a total reaction volume of 20 µL. Over the course of 1 hour, the data show similar fluorescence activation. However, the homemade reaction begins to saturate after 1 hour, likely due to the exhaustion of NTPs in the homemade reaction (11.4 mM) when compared to the commercial kit (40 mM). All data shown for n=3 independent biological replicates as lines with raw fluorescence values standardized to MEF (µM FITC). Shading indicates the average value of 3 independent biological replicates ± standard deviation.