Figure 3. Methylation of reporter transcripts in human cells.

(a) HEK293T cells were transfected with plasmids encoding a TRM editor, Cas13 guide RNA, and the targeted Cluc reporter transcript containing a 3’ UTR methylation target. (b) Methylation of a synthetic 3’ UTR fused to Cypridina luciferase (Cluc–syn) by TRM editors. (c) Methylation of the Socs2 3’ UTR fused to Cypridina luciferase (Cluc–Socs2) by TRM editors. Inactive TRM editors contain a methyltransferase-inactivating D395A mutation within M3. Values and error bars reflect the mean±s.e.m. of n=3 independent biological replicates.