Figure 6. TRM effect on RNA abundance and alternative splicing.

Differential RNA expression of HEK293T cells transfected with (a) dCas13–M3nls or (b) dCas13–M3M14nes and either Actb A1216-targeting or non-targeting guide RNAs. The targeted Actb gene is marked in red. Inactive TRM control indicates editors containing a methyltransferase-inactivating M3 D395A mutation with an Actb-targeting guide RNA. Non-target gRNA indicates methyltransferase-active TRM editors with a non-targeting guide RNA as a control. Differential RNA-seq analysis was performed with n=5 independent biological replicates. Statistical significance was calculated using a two-tailed Student’s t-test with false discovery rate correction. Alternative splicing of (c) Brd8 and (d) Znf638 in HEK293T cells transfected with dCas13–M3nls and the indicated guide RNAs (purple) for targeting m⁶A sites shown (red). Exon exclusion or inclusion indicates the percentage of RNA isoform lacking or containing the alternatively-spliced exon, respectively. Values and error bars reflect the mean±s.e.m. of n=3 (c) or n=6 (d) independent biological replicates. P values were calculated using a two-tailed Student’s t-test.