Fig. 2.

Elevated glucosuria but no enhancement in beta cell function post glucose administration in obese male Mc4r null 24-week-old C57Bl/6 mice. (a) Blood glucose levels during the IVGTT (mice were fasted for 6 h before the test); n=7 WT and n=6 Mc4r^−/−, (b) plasma insulin levels during IVGTT; n=7 (c) 24 h urine glucose concentration at baseline, (d) 24 h urine glucose concentration after administering 250 mg glucose by oral gavage, (e) 24 h urine glucose concentration after administering 200 mg glucose by i.p. injection. Two-tailed unpaired Student’s t test or repeated measures two-way ANOVA followed by Bonferroni’s multiple comparison test were used for comparisons. **p<0.01, ***p<0.001 vs WT; ^†p<0.01 vs 0 min, WT. Error bars are mean ± SEM. Mc4r^−/−, Mc4r^loxTB/loxTB