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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Cancer Res. 2020 Oct 6;80(23):5344–5354. doi: 10.1158/0008-5472.CAN-19-0677

Fig. 5. SEL decreases FANCD2 and mono-ubiquitination FANCD2.

Fig. 5.

Human U266 (A), 8226 (C) and MEL-resistant U266-LR6 (B) 8226-LR5 (D) MM cells were treated for 2 hours with 50 μM MEL, washed, and placed in fresh media with or without control or SEL. Protein (100 μg) was separated by gradient acrylamide gel electrophoresis for 4 hours to separate FANCD2 from mono-ubiquitinated-FANCD2. SEL was found to decrease both ubiquitinated and total FANCD2 as shown in representative Western blots from both parental U266 (A) and 8226 (B) and MEL-resistant U266-LR6 (C) and 8226-LR5 (D) cells(n=4).