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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Cancer Res. 2020 Oct 6;80(23):5344–5354. doi: 10.1158/0008-5472.CAN-19-0677

Fig. 6. SEL inhibits MEL-induced mono-ubiquitination of FANCD2 in both parental and drug-resistant 8226 human MM cells.

Fig. 6.

8226 and 8226-LR5 human MM cells were either untreated or treated with 50 μM MEL for 2 hours. The cells were then collected by centrifugation and incubated for 24 hours with fresh media with or without 300 nM SEL. The cells were assayed for proximity co-localization of FANCD2 and ubiquitin. More than 700 cells were assayed for each experimental treatment group. The proximity ligation assay generates a red fluorescent signal when the two proteins tested (FANCD2 and ubiquitin) are within 40 nM of each other. The values on the y-axis represent the change in ubiquitin-FANCD2 nuclear foci percentages compared to untreated controls (whose values were set at 100%).