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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Cancer Res. 2020 Oct 6;80(23):5344–5354. doi: 10.1158/0008-5472.CAN-19-0677

Fig. 7. FANCD2 siRNA knockdown prevents DNA repair and increases DNA damage (γH2AX protein expression) in the presence of MEL in both parental and MEL-resistant human MM cells (n = 4).

Fig. 7.

Human MM U266 and MEL-resistant U266-LR6 cells were transfected with control (scram) siRNA and FANCD2 siRNA. After 48 hours, cells were treated with 50 μM MEL for 2 hours, washed, and then incubated for a further 48 hours. At the 24- and 48-hour time points, DNA damage was assessed by measuring γH2AX protein expression by FACS analysis. FANCD2 knockdown was > 82% (U266 cells inset).