Figure 1.

Validation of a Leading Wnt Inhibitor

(A) Synthesis of CBA-1 from 5-chloroquinolin-8-ol (1 eq), butyramide (4 eq), and 4-diethylaminobenzaldehyde (1 eq), 130°C, 2 h followed by the addition of isopropanol to induce precipitation (71% yield).

(B) Effects of CBA-1 (3 μM) and XAV939 (5 μM) on TOPFlash reporter activity (∗p < 0.0001; #p < 0.01; n = 3).

(C) CBA-1 repressed Wnt target genes expression in LS174T CRC cells. Densitometry was analyzed using three western blot images (∗p < 0.05; n = 3).

(D) CBA-1 inhibited CRC cell proliferation (∗p < 0.001; #p < 0.05; n = 3).