Figure 2.
CBA-1 Regulates Histone Methylation
(A) CBA-1 did not inhibit the binding between β-catenin and TCF4. FLAG-tagged β-catenin and HA-tagged TCF4 were transfected separately into HEK293T cells. Equal amounts of β-catenin and TCF4 were mixed and immunoprecipitated by an anti-FLAG antibody. TCF4 was analyzed by an anti-HA antibody.
(B and C) CBA-1 significantly increased H3K9Me2 and H3K27Me3 in HEK293T and LS174T cells. Densitometry was analyzed using three western blot images (∗p < 0.05; n = 3).to new sentences: (B) CBA-1 significantly increased H3K9Me2 and H3K27Me3 in HEK293T cells. Densitometry was analyzed using three western blot images (∗p < 0.05; n = 3). (C) CBA-1 significantly increased H3K9Me2 and H3K27Me3 in LS174T cells. Densitometry was analyzed using three western blot images (∗p < 0.05; n = 3).
(D) CBA-1 increased H3K9Me2 on the promoters of Wnt target genes. The promoter sequences were analyzed by both standard PCR (left) and qPCR (right) (∗p < 0.01; n = 3).
(E) KDM3A, a potential target of CBA-1, overexpressed in CRC. Tumor: red. Normal tissue: gray.
(F) High levels of KDM3A correlated with poor CRC patient survival.