Figure 8.

Subpopulations of SMG ID Are Defined by Expression of Smgc, Gstt1, and Gfra3

(A) Immunofluorescence staining showing localization of Smgc (green) in P1 and P30 SMG. Proacinar and acinar cells are labeled with Mist1 and Aqp5 (red).

(B) qPCR for genes enriched in Smgc+ (top panel) and Kit+ ID cells (bottom panel). Data are normalized to Rsp29 and age-matched female SMG (dotted line). Data are represented as mean ± SEM, and asterisks show statistical significance (p < 0.05) compared with age-matched female controls (n = 3; two-tailed t test).

(C) Immunofluorescence staining showing expression of Gstt1 (red) in Smgc+ cells (green, top panel) and Nkcc1+ cells (green, lower panel) in P30 SMG from male and female mice.

(D) UMAPs showing selected DEGs in Kit+ ID cells in P30 glands.

(E) Immunofluorescence shows distinct ID populations with no overlap between Gfra3, Gstt1, and Krt14 protein.

(F) In situ hybridization showing expression and localization of Gfra3, Prol1, Nkd2, Aqp5, and Esp18 mRNA in P30 SMG. Scale bars, 20 μm.