Fig. 3.

Effects of Na₂S₄ on EMT process in HK-2 cells. HK-2 cells were pre-incubated with Na₂S₄ (30 μM) for 30 min, and then challenged by or HG (30 mM) for 48 h. (A–C) Representative blots and quantitative analysis of TGF-β1, α-SMA, E-cadherin showing that Na₂S₄ (30 μM) mitigated HG-induced fibrosis in HK-2 cells. (D–F) Relative mRNA levels of TGF-β1, α-SMA, E-cadherin. (G) Immunofluorescence staining for E-cadherin or α-SMA. Scale bar = 200 μm *P < 0.05 vs. NG. †P < 0.05 vs. Vehicle (Veh). n = 4 to 6.