The effect of feeding schedule and isoflurane anaesthesia on rest-activity rhythm during 7 days (D1, D2, D3, D4, D5, D6, D7). (a–d) Representative double-plotted actograms show the activity patterns after feeding schedule and isoflurane anaesthesia in a 12 h:12 h light:dark cycle. The black sections in the actograms represent a greater amount of activity than the average value in 48 h, while the white ones represent less activity, the abscissa 8.00 means ZT0; 20.00 means ZT12. Pentagram represents anaesthesia administration from ZT14 to ZT19. (e) Phase shift of acrophase. (f) Amplitude. (g) MESOR. (h) Diurnality score. a
P < 0.05 vs mean values in 7-day pre-administration period; b
P < 0.05 vs Control + Anes group; c
P < 0.05 vs Night-Feeding group; d
P < 0.05 vs Night-Feeding + Anes group; e
P < 0.05 vs Day-Feeding group, n = 8.