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. 2020 Dec 5;39:273. doi: 10.1186/s13046-020-01722-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Screening and identification of anti-MUC18 mAb. Binding of JM1-24-3 to its target on melanoma cell surface is verified by FACS and ELISA a FACS analysis of JM1-24-3 binding on the surfaces of live melanoma cells showed that high metastatic cell lines A2058 and WM266-4 had significantly higher binding compared to low metastatic cell line A375. b ELISA analysis of JM1-24-3 binding with the immobilized melanoma cell lysates showed similar binding pattern as of FACS. High metastatic cell line A2058 showed the maximum binding. MUC18 protein is defined as the target of JM1-24-3 by immunoprecipitation (IP) and mass spectrometry (MS) analyses c Immunoprecipitation of WM266-4 lysates with JM1-24-3 showed two bands - 135kD (high intensity) and 110kD (faint band) by SDS-PAGE Coomassie blue staining. d MS analysis showed that MUC18 glycoprotein had invariably the highest score among all the hits for both the bands