Figure 5.

Metabolic output of S2R+ cells grown in FBS- or FEx-supplemented media are similar. (A) Volcano plot of metabolites comparing FBS-supplemented cell-free media vs. FBS-supplemented culture supernatant. The metabolites that are uniquely changing in FBS-supplemented culture supernatant are labeled in red (B) Volcano plot of metabolites comparing cell-free media vs. 2.5% FEx-supplemented culture supernatant. The metabolites that are uniquely changing in FEx-supplemented culture supernatant are labeled in green. Metabolites that are significantly (P < 0.05) changed when the S2R+ cells were grown in cell-free media vs. the respective FEx- or FBS-culture supernatant by at least twofold are highlighted in pink. Metabolites that are pink but unlabeled or labeled in blue in either panels change similarly irrespective of the media being supplemented with FBS or FEx. Nicotinamide, the only metabolite to display opposing changes depending on whether the cells were cultured in FEx or FBS is labeled in black.