Figure 5.

Ubiquitous knockdown of the PDCD2L fly ortholog trus causes lethality but does not affect germ cell specific development. A) Developmental rate and B) GFP+ rate (RNAi element absent) of individuals from each line crossed to Tub-Gal4 with GFP balancer. Fertility was tested in males with germ cell-depleted trus levels, using Nanos (C) and Bam (D) drivers, through counting pupae generated after mating with w¹¹¹⁸ females. Each data point represents a single vial counted. ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to w¹¹¹⁸ at corresponding developmental stage. E) Knockdown efficiency of each trus RNAi line as measured by quantitative PCR, normalized to RpL23. ** P < 0.01, *** P < 0.001 compared to wildtype.