Skip to main content
. 2020 Oct 14;10(12):4449–4457. doi: 10.1534/g3.120.401714

Table 4. Fly lines used in this study and their genotypes.

Line Purpose Genotype Source
w1118 Wildtype control w1118 Bloomington, line 3605
GFP RNAi Non-specific RNAi control w[*]; P{w[+mC]=UAS-GFP.dsRNA.R}143/CyO; Sb[1]/TM6B, Tb[1] Bloomington, line 44412
Musashi (msi) RNAi Infertile control UAS-dicer; UAS-msi.dsRNA / [SM6B - TM6B] Gary Hime lab, The University of Melbourne
CLC-B RNAi Viable RNAi control w1118; P{KK100840}VIE-260B Vienna Drosophila Resource Centre, stock 103420
trus RNAi 1 Gene of interest knockdown w1118; P{GD11610}v22066 Vienna Drosophila Resource Centre, stock 22066
trus RNAi 2 Gene of interest knockdown w1118; P{GD11610}v22067 Vienna Drosophila Resource Centre, stock 22067
Nanos-Gal4; UAS-Gal4 Germ cell conditional driver, UAS drives maximal knockdown capacity w[*];P{w[+mC]=UAS-GAL4}; P{w[+mC]=GAL4-nos.NGT}A Gary Hime lab, The University of Melbourne
Bam-Gal4 Germ cell conditional driver y[1] w[*] P{w[+mC]=bam-GAL4:VP16}1 Bloomington, line 80579
Tubulin-Gal4 with GFP balancer Body-wide driver Tub-GAL4/TM3, Sb, twi-GAL4, UAS-GFP In-house, derived from Bloomington line 6663
Tubulin-Gal80ts; Tubulin-Gal4 Body-wide driver paired with temperature sensitive repressor w[*]; P{w[+mC]=UAS-3xFLAG.dCas9.VPR}attP40, P{w[+mC]=tubP-GAL80[ts]}10; P{w[+mC]=tubP-GAL4}LL7/TM6B, Tb[1] Bloomington, line 67065