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Diagram of the CSS backcross strategy to map epistatic QTL pairs. (A) (B6.A4 x B6)F1 mice were crossed with (B6 x B6.A6)F1 mice to generate 149 “N2” offspring. (B) Seven hypothetical “N2” offspring are shown. The recombinant chromosomes 4 and 6 are derived from A/J or C57BL/6J as indicated. All other chromosomes are B6- derived in all mice and therefore lack any allelic variation in this cross.
