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. 2020 Oct 27;10(12):4665–4678. doi: 10.1534/g3.120.401661

Figure 1.

Figure 1

Growth and viability of yeast under MCHM treatment. A. Growth of 10-fold dilutions of yeast on YPD media with and without MCHM treatment. BY4741 strain is derived from the S288c background. B. Acute viability assay of the MCHM sensitive strain BY4741 treated with MCHM for 1.5 hr and 24 hr. Colony-forming units for three biological replicates were averaged for each treatment then normalized to media without MCHM treatment. In YPD yeast were exposed to 400ppm and 550ppm crude MCHM. C. Proportion of log-phase BY4741 in G1, S, or G2/M phases of the cell cycle based on flow cytometric analysis grown in YPD. D. Histograms of DNA content in BY4741 population grown in YPD shown in part C, as based on fluorescence intensity of sytox green. E. The proportion of log-phase BY4741 in G1, S, or G2/M phases of the cell cycle based on flow cytometric analysis grown in YPD with 550ppm MCHM. F. Histograms of DNA content of BY4741 population grown in YPD treated with MCHM shown in part E, as based on fluorescence intensity of sytox green. All samples were analyzed for 0, 0.5, 1.5, 6, and 12-hour time points to monitor cell cycle changes over time.