Figure 6.

Measurement of changes in mRNA and protein expression of HDAC 3. Measurement of changes in mRNA and protein expression of HDAC 3 by SNC-121 treatment in normal and ocular hypertensive animals at day 7 (a, c) and day 42 (b, d) post-injury. Total RNA was extracted from retina using TRIzol reagent and 1 µg RNA was used to synthesize cDNA. The mRNA levels were examined by quantitative RT-PCR using primers specific to HDAC 3 and relative gene expression was calculated based on the comparative threshold cycle (Ct) method, normalized to housekeeping genes, β-actin a and b. Retina lysates (20 µg) were analyzed by Western blotting using anti-HDAC 3, and anti-β-actin antibodies. The band intensities were measured using chemiluminescent reagent and Versadoc imaging system c and d. Data expressed as mean ± SE. **P < 0.01; ***P < 0.001; ****P < 0.0001; n = 3–23.