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. 2020 Dec 4;11:6226. doi: 10.1038/s41467-020-20026-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a MALDI-TOF/TOF spectra of TS_pHYSIMAR spiked in BSA digests at 1:100 (m/m) before and b after enrichment by SiO₂@DpaZn. Triangle indicates the N-phosphopeptide, and asterisk indicates the dephosphorylated counterpart TSHYSIMAR. c Degradation curve of N-phosphopeptide in vortex and ultrasound treatment under neutral condition. Each represents the mean from n = 3 parallel experiments, and the mean represents recovery of phosphopeptide. The error bar is represented by SD. d Adsorption kinetics of SiO₂@DpaZn. Each value represents the mean from n = 3 parallel experiments, and mean represents adsorption capacity of SiO₂@DpaZn. The error bar is represented by SD.