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. 2020 Dec 4;10:21293. doi: 10.1038/s41598-020-77986-z

Figure 5.

Figure 5

Proton uncaging changes the S4 conformation in closed state. (A) left, cartoon depicting ciHv1-245C-TAMRA and chemical structure of NPE-caged-proton at the intracellular side. Right, scheme of the outside-out PCF recording condition with NPE-caged-proton in the pipette. (B) representative outside-out PCF recording of ciHv1-L245C-TAMRA held at − 80 mV, and 1 s UV-light exposure at time points indicated by bars. Horizontal lines (dotted = pre UV, dashed = after UV) indicate the fluorescence at − 80 mV. (C) mean Fsignals in response to repetitive voltage steps from − 80 mV to − 5 mV before UV (left) and after UV (right). (D) fluorescence ratio, after and before 1 s UV-light exposure, at − 80 mV (0.9 ± 0.07; n = 3 different patches from 3 different cells). (E) representative outside-out PCF recording of ciHv1-L245C-TAMRA without NPE in the pipette, held at − 80 mV, and 1 s UV-light exposure at time point indicated by bar. Error bars indicate the SD.