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. 2020 Dec 4;10:21248. doi: 10.1038/s41598-020-78057-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Diversity comparison of SC, CF and RC microbiota. (A) Boxplot of ACE and Chao 1 diversity in the three groups. Outliers are represented by dots. Significant differences between groups are shown by lines and the following notation. P < 0.05 (*), P < 0.01 (**), P < 0.001 (***). (B)Venn diagram illustrated the number of shared OTUs between SC, CF and RC at 97% similarity. Coloured circles represented each group and intersection part between circles represented the number of shared OTUs. (C) Principal coordinates analysis (PCoA) based on weighted UniFrac distance of community structure among all the individuals of three treatment groups. Each color circle represents one sample: pink for SC group (n = 10); blue for CF group (n = 8); violet for RC group (n = 10). (D) Box plot comparison of beta diversity analysis across treatment groups at genera level. The X-axis denotes the population studied while Y-axis denotes the corresponding Shannon–Weaver index representing the beta diversity of treatment and gender group.