Table 1.
LAMP primer and amplicon sequences (gBlock) and parameters. The F2 and B2 primer regions of FIP and BIP are underlined.
| LAMP primer or amplicon | Sequence 5′–3' | Primer length (bp) | Predicted Tm, annealing temperature (°C) | Degeneracy of primer (fold) |
|---|---|---|---|---|
| Phylloxera gBlock fragment | cccGGAGCAATTAATTTTATTTGTACTATTAcccCCAAATAATATAAAATTAAATCAAATCCcccCATTATTCCCCTGGTCAATTTTcccCAGCTATTTTATTAATTTTATCTTTAcccCCTGTATTAGCTGGTGCTATTACAcccCAGATCGTAACTTAAATACATCcccATCCATCAGGAGGAGGGGcccATCCAGTTTTATACCAACACCTATTTccc | 221 | N/A | None |
| Phy-F3 | GGAGCAATTAATTTTATTTGTACYATTA | 28 | 58.2 | 2 |
| Phy-B3 | AAATARRTGTTGRTATAAAACTGGAT | 26 | 59.8 | 8 |
| Phy-FIP | TAAAGATAAAATTAATAAAATAGCTGCCAAATAATATAAAATTAAATYCAAATCC | 55 | 68.5 | 2 |
| Phy-BIP | CCYGTATTARCTGGTGCTATTACACYCCTCCTCCTGATGGAT | 42 | 75.3 | 8 |
| Phy-FL | AAAATTGAYCAGGGGAATAAYG | 22 | 62.2 | 4 |
| Phy-BL | CAGATCGTAAYTTAAATACATC | 22 | 57.7 | 2 |