Table 3.
Performance of the phylloxera non-destructive crude DNA samples from three life stages tested for application in LAMP, real-time qPCR, microsatellite genotyping, and DNA barcoding.
| Specimens† | n | LAMP | qPCR (Cq value) | Microsatellite | DNA barcoding | ||
|---|---|---|---|---|---|---|---|
| Amplification time (min) | Anneal derivative temperature (°C) | 5 µl DNA | 2 µl DNA | ||||
| Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | (genotype) | (match on BOLD) | ||
| Egg | 3 | 14.0 ± 2.6 | 80.5 ± 0.6 | 22.7 ± 3.5 | 24.7 ± 2.7 | G4 | D. vitifoliae,100% |
| Crawler | 3 | 15.8 ± 0.0 | 79.1 ± 0.1 | 25.3 ± 1.1 | 26.3 ± 1.0 | G4 | D. vitifoliae,100% |
| Adult | 3 | 18.6 ± 3.8 | 78.7 ± 0.2 | 23.9 ± 3.6 | 25.4 ± 3.6 | G4 | D. vitifoliae,100% |
†G4.