Fig. 1. Twenty DNA origami cryo-EM solution structures drawn to scale.

All maps were determined in freestanding ice. a Four 48-helix Brick DNA origami objects. Left to right: 0, 1, 2, 4 unpaired thymidines added at each staple crossover, respectively. b A barrel-like 126-helix bundle DNA origami object one-pot assembled with two scaffold chains. c Two design variants of a multidomain square-lattice DNA origami called Twisttower. d A design variant (v2) of the previously reported Pointer¹³. e A 16-helix bundle DNA origami object assembled from a 1317 base-pair long-scaffold strand. f Four variants of a previously described hinged-beam-like object²³. g Two variants of a dumbbell-like DNA origami object with a crossover-free handle segment. h Four six-helix bundle DNA origami objects, two with one asymmetric feature at one end (bottom), two with two asymmetric features at each end. Bottom right: a ten-helix bundle with one asymmetric feature in the middle. See Supplementary Figs. S1–20 for more cryo-EM imaging details. Strand diagrams for each object are supplied in Supplementary Figs. S43–S63.