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. 2020 Dec 4;11:6229. doi: 10.1038/s41467-020-20020-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Left: exemplary region of interest in the 4 × 4 domain in the Twisttower cryo-EM map. Right: same region, after two-body refinement using the two bodies indicated in the middle. b Schematic illustration of the iterative application of the procedure in (a) over the entire object. Bottom: Frankenstein map, created by combining the focal elements. c–f Left to right: arrows give examples of high-resolution features revealed after scanning-focused refinement: single-strand phosphate backbone bond, double-strand phosphate backbone bond, depression at a strand-break site, peripheral single-strand crossovers, and density from poly-T tails at a helical interface.