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. 2020 Dec 4;11:6229. doi: 10.1038/s41467-020-20020-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic illustration of the cascaded relaxation procedure. Markers along the arrow represent secondary restraints applied to the structure: interhelical (orange, vertical springs), intrahelical (blue, horizontal springs), and hydrogen bonds of the Watson–Crick pairs (H–O). b Atomic models derived from fitting to cryo-EM maps: Twisttower (overlay with map), Pointer-v2, 126-helix bundle, 16-helix bundle, 48-helix brick, and twist-corrected Twisttower. Scaffold atoms are depicted in gray, staple strands in color.