Fig. 3.

TGFBI levels determine tumour hypoxia. (A) cibersort analysis of MMTV‐PyMT;Tgfbi ^+/+ and MMTV‐PyMT;Tgfbi ^Δ/Δ. (B) The presence of CD45⁺CD11b⁺ cells was determined by FACS in fresh MMTV‐PyMT;Tgfbi ^+/+ and MMTV‐PyMT;Tgfbi ^Δ/Δ tumours. Data were analysed by unpaired t‐test (n = 12 wt, n = 10 ko). (C) FACS analysis of CD8⁺ T cells in fresh MMTV‐PyMT;Tgfbi ^+/+ and MMTV‐PyMT;Tgfbi ^Δ/Δ tumours. Data were analysed by Mann–Whitney's test (n = 19 wt, n = 20 ko). (D) Bone marrow transplantation experimental scheme. GFP⁺ bone marrows from Tgfbi ^+/+ or Tgfbi ^Δ/Δ mice were transplanted into lethally irradiated wild‐type hosts. The resultant chimeras were orthotopically injected with MMTV‐PyMT;Tgfbi ^Δ/Δ tumour cells. The content of CD24⁺CD90⁺ (E) in these tumours was analysed by FACS. Data were analysed by unpaired t‐test, and are presented as mean and SD (n = 5 wt, n = 4 ko). (F) PIMO staining in Tgfbi ^+/+ and Tgfbi ^Δ/Δ bone marrow transplanted mice tumours and quantification of stained area (scale bar 100 μm). Data were analysed by unpaired t‐test, and are presented as mean and SD (n = 3). (G) Tgfbi, Rgs5, and Hif1α qPCR on pulverised tumour material from Tgfbi ^Δ/Δ chimeras. Data were analysed by unpaired t‐test, and are presented as mean and SD (n = 5). Rplp0 was used as a housekeeping gene. BM, bone marrow; BMT, bone marrow transplantation; PIMO, pimonidazole. *P < 0.05; ***P < 0.001; n.s., not significant.