Fig. 6.

Blocking PPARγ reverses the effects of FABP12 on cell migration and Slug induction. (A, B) The scratch assay shows that treatment with the PPARγ inhibitor GW9662 reduces cell motility (increased relative open area) in DU145 cells transiently transfected with pcDNA3.1‐HA‐FABP12 compared to control transfectants (A) and PC3 cells stably transfected with pREP4‐FABP12 compared to control transfectants (B). Two‐way ANOVA was used for statistical analysis. (C, D) The Transwell assay shows that treatment of stable PC3‐pREP4‐FABP12 transfectants with PPARγ inhibitor (GW9662) reduces cell migration. Images are shown in (C) and cell counts in (D). Statistical analysis was carried out using Student's t‐test. (E, F) Western blot showing reversal of Slug induction in PC3‐pREP4‐FABP12 cells upon PPARγ inhibition with GW9662 (E) or PPARγ depletion using two different siRNAs (F). **P < 0.01. N = 3. Error bar: SD.