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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Transl Res. 2020 Jun 15;227:1–14. doi: 10.1016/j.trsl.2020.06.006

Fig 5.

Fig 5.

MBX-8025 restores gut barrier function during chronic ethanol feeding. C57BL/6 mice were fed an oral isocaloric (control) diet (1–2 technical replicates) or a chronic Lieber-DeCarli diet for 8 weeks followed by 1 binge of ethanol (3–4 technical replicates), and treated with vehicle, or MBX-8025 (10 mg/kg/d) daily by adding MBX-8025 in the liquid diet during the entire feeding period (prevention) or during the last 4 weeks of ethanol feeding (intervention). (A) Fecal albumin. (B) Serum LPS. (C) Ranking of top 10 biological processes by −log(P value) in the ileum of MBX-8025 treated (prevention group) compared with vehicle treated, ethanol-fed mice. (D) Ranking of top 10 biological processes by −log(P value) in the ileum of MBX-8025 treated (intervention group) compared with vehicle treated, ethanol-fed mice. (E) Ileal Dhrs9 mRNA level. (F) Ileal Ftcd mRNA level. (G) Ileal FoxMl mRNA level. (H) Ileal Sox9 mRNA level. (I) Ileal S100G mRNA level. (J) Ileal Mgl2 mRNA level. Control diet: Vehicle, n = 10; MBX-8025/Prevention, n = 9–10; Ethanol diet: Vehicle, n = 15–19; MBX-8025/Prevention trial, n = 21–23; MBX-8025/Intervention, n = 17–22. Results are expressed as mean ± s.e.m (A, B, E–J). P values were determined using one-way ANOVA with Tukey’s post hoc test for ethanol-fed mouse groups and Mann-Whitney-Wilcoxon test for control diet-fed mouse groups (A–C, F–K). *P < 0.05, **P < 0.01, ****P < 0.0001.