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Schematic view of dimeric heme-containing oxygenase domain and FMN-, FAD- and NADPH-binding (sub)domains in nNOS holoprotein. The two nNOS splice variants, nNOSα and nNOSµ, are both full-length proteins, which only differ in the number of amino acids in the autoregulatory insert within the FMN domain: nNOSµ contains extra 34 residues, compared to nNOSα. CaM binds to the linker connecting the FMN and heme domains, while Hsp90 associates with the heme domain.
