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The heme blocker imidazole inhibits the O2•− production in Hsp90α associated nNOSµ (left panel) or nNOSα (right panel) protein under l-Arg depleted conditions. 5 mM imidazole was added into the reaction system. The experimental conditions were the same as described in Figure 2. EPR spectra were recorded continuously, and the spectra presented here were collected 15 minutes after adding NADPH into the reaction system.
