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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Inorg Biochem. 2020 Nov 4;214:111298. doi: 10.1016/j.jinorgbio.2020.111298

Table 1.

NADPH oxidation rates of nNOS proteins under various conditions a

NADPH oxidation (min−1)
nNOSµ 78.4 ± 0.6
nNOSµ + l-Arg 45.4 ± 5.1
nNOSµ + Hsp90α 86.8 ± 1.1
nNOSα 100.1 ± 0.6
nNOSα + l-Arg 30.9 ± 0.6
nNOSα + Hsp90α 119.8 ± 10.2
a

Initial rates of oxidation of NADPH by nNOS were determined in a pH 7.4 buffer containing 50 mM Tris, 100 mM NaCl, 200 µM CaCl2, and 100 µM NADPH. The final concentrations of nNOS and CaM were 20 nM and 1 µM, respectively. The concentrations of l-Arg and Hsp90 protein in the assay mixture, if added, were 100 µM and 3 µM, respectively.