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. 2020 Dec 5;31(12):132. doi: 10.1007/s10856-020-06462-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Different calcification modes in decellularized heart valves: A Von Kossa stain showed comparison between SPV Glu-fixed heart valves (upper image), porcine matrix (middle) and decellularized Kangroo matrix showing lowest calcification in the SPV samples for calcium in explant [75], B Von Kossa staining of (B-1) trypsin, (B-2) osmotic, (B-3) trypsin-osmotic, and (B-4) detergent-osmotic treated matrices seven days after implantation. Calcific deposits are only present in trypsin matrices (B-1), as indicated by black staining [49], C Pre-implantation histologic for model 700 SynerGrafte heart valves showed multiple calcific deposits within the matrix [81]