Fig. 3.

Expanded G4C2r expression increases the levels of specific forms of phosphorylated tau protein in Drosophila and in human cells. A Representative western blots and quantification of brain lysate samples from indicated genotypes showing that the phosphorylated tau levels detected by the antibodies AT8, pS262, and pS199 were increased by (G4C2)₃₀ expression in the adult fly head, compared to controls expressing (G4C2)₃ (i, ii, iii) (numbers in bars, numbers of biological replicates; statistical analysis, two-tailed unpaired t test). B As in A, but in transfected HEK293T cells. In all plots, bars indicate the mean ± SEM; *P ≤ 0.05, **P ≤ 0.01.