Figure 2.

TRIM35 expression is induced by viral infection and IFN-β treatment. (A–C) IB analysis of TRIM35 expression in mouse peritoneal macrophages (A), A549 (B), or HEK293T (C) cells infected with WSN (H1N1) virus (MOI = 3) for 20 h. MPM, mouse peritoneal macrophages. (D–F) IB analysis of TRIM35 expression in mouse peritoneal macrophages stimulated with poly(I:C) (D) or 5′-pppRNA (E) or were infected with SeV (F) at the indicated timepoints. (G) IFA for TRIM35 expression in Hela cells that were mock infected or infected with SeV for 8 h. Scale bars, 20 μm. (H) IB analysis of TRIM35 expression in mouse peritoneal macrophages treated with the indicated amounts of IFN-β for 12 h. The interferon-induced transmembrane protein 3 (IFITM3) was included as a positive control. Data are representative of at least three independent experiments